RtMastitisTM PCR Test

The RtMastitis test fits well with other services offered by DTS, particularly with the Farmer Payment Testing Services and Herd Improvement Program testing. The RtMastitis PCR test uses real-time PCR (RtPCR) technology to rapidly identify mastitis-causing bacteria from bovine milk.


Samples received at DTS by 8am can have results available on the same day, providing a potential 24hr turnaround from sampling on farm to result.


The RtMastitis PCR test can be used for analysis of fresh and bronopol preserved bovine quarter milk samples, herd recording samples and bulk tank samples. DTS Food Laboratories can provide sample tubes containing bronopol, which eliminates bacterial growth, enabling room temperature shipping.


Bacterial DNA from live, growth inhibited or dead bacteria can be detected, which provides results for many samples that would otherwise show 'no growth' in conventional bacterial culture. Simultaneous detection of all targeted bacteria present in the milk sample overcomes the common problem of 'mixed growth' results of bacterial culturing.

Testing options

RtMastitis major-3 PCR test: precise identification of three key contagious mastitis-causing bacteria:

  • Staphylococcus aureus
  • Streptococcus agalactiae
  • Mycoplasma bovis

RtMastitis complete-12 PCR test: precise identification of the most common mastitis-causing bacteria and the penicillin resistance gene:

  • Streptococcus uberis
  • Staphylococcus aureus
  • Staphylococcus sp.
  • Beta-lactamase penicillin resistance gene
  • Streptococcus agalactiae
  • Streptococcus dysgalactiae
  • Escherichia coli
  • Enterococcus sp.
  • Klebsiella sp.
  • Corynebacterium bovis
  • Serratia marcescens
  • Arcanobacterium pyogenes and/or Peptoniphilus indolicus